Pi³ Technology

The Pi³ technology provides an easy self-contained method, which uses a novel water-insoluble reagent that forms a covalent bond with amino acid specific peptides. The method allows for the selective separate and isolate peptides containing specific amino acids from peptide mixtures. These separated amino acid specific peptides subsequently may be regenerated and analyzed by mass spectrometry or liquid chromatography. Use of the mass spectrometric data and the method of digestion, when coupled with the added constraint that the peptides contain specific amino acids, can lead to improved accuracy in the identification of proteins. Chromatographic analysis of the amino acid specific peptides containing peptides can simplify complex separations.

The Method

The method employed for the use of the Pi³ Reagent consists of 5 easy steps using standard laboratory reagents and equipment:

1. Capture the amino acid specific peptides on the solid-support.
2. Wash the support to remove any non-bound peptides.
3. Release the bound peptides.
4. Collect the released peptides and wash the solid-support.
5. Desalt and concentrate the peptide solution prior to analysis.

The Benefits

• Specific isolation
• Isolation based on specific functionality
• Covalent attachment to activated solid-support
• Efficient wash removes non-specific adsorption
• Releasing reagent easily cleaves covalent bond
• Improve and simplify mass spec data base search accuracy
• Complex sample mixtures are simplified
• Low-level components are more easily identified
• Multi-component mixtures are more easily determined
• Presence or absence of specific functionality can be ascertained
• Ready to use kit
• No methods development or optimization
• Uses common laboratory reagents
• Requires only vortex mixer and mini-centrifuge
• Higher Productivity
• Significant time and money savings
• Multiple samples may be run concurrently

Contact

Email: info@mlwtechnologies.com